Microbial Assay of antibiotics & Antibiotic potency test
Antibiotic Microbial Assays Cylinder-Plate (Agar Diffusion) and Turbidimetric (Tube) Methods Antibiotic microbial assays are used to determine the potency of antibiotics by measuring their effect on bacterial growth. Two main methods are used: Cylinder-Plate (Agar Diffusion) Method Turbidimetric (Tube) Method 1. Cylinder-Plate (Agar Diffusion) Method The Cylinder-Plate Method (also called the Agar Diffusion Method ) is widely used in antibiotic potency testing because it directly measures how well an antibiotic diffuses through a solid medium (agar). This property is crucial because an antibiotic must spread effectively to inhibit bacterial growth at a distance from the source. 1. Understanding Diffusion in Agar Diffusion refers to how a substance spreads from a higher concentration to a lower concentration within a medium. In the cylinder-plate method , antibiotics diffuse from a central point (cylinder or well) into the agar, creating a concentration g...